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PH 6?1 (Amersham Biosciences) for cup loading (pI 6?). Strip loading Rehydration loading was applied for strips of pI 4? with the recommended volumes, while strips of pI 6? were cup-loaded in a volume of 20 ?50 after rehydrating the strips over night with rehydration buffer for basic strips (7 M Urea, 2 M Thiourea, 4 Chaps, 5 /ml IPG buffer pH 6?1 (Amersham Biosciences), 12 /ml Destreak (Amer
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R 300 V to 10,000 V, 10,000 V for 105 kVhrs, 500 V till proceeding to 2nd dimension.Page 7 of(page number not for citation purposes)BMC Developmental Biology 2006, 6: http://www.biomedcentral.com/1471-213X/6/2nd dimension Strips were reduced (20 mg/ml DTT in equilibration buffer: 6 M Urea, 2 SDS, 30 glycerol, 0.375 M Tris pH8.8, 0.002 bromphenol blue) and alkylated (25 mg/ ml Iodoacetamide in
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R 300 V to 10,000 V, 10,000 V for 105 kVhrs, 500 V till proceeding to 2nd dimension.Page 7 of(page number not for citation purposes)BMC Developmental Biology 2006, 6: http://www.biomedcentral.com/1471-213X/6/2nd dimension Strips were reduced (20 mg/ml DTT in equilibration buffer: 6 M Urea, 2 SDS, 30 glycerol, 0.375 M Tris pH8.8, 0.002 bromphenol blue) and alkylated (25 mg/ ml Iodoacetamide in
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Threshold). 8 Peptides matched to identified protein/ fingerprint masses searched. 9 Sequence coverage of the matched peptides. 10 (R)oot (M)ean (S)quare error in ppm of predicted versus apparent peptide mass (m/z). 11 Database. 12 Corresponding accession number of identified proteins. 13 Link to sequences and online resources (Ensembl and TIGR only). Click here for file [ http://www.biomedcentral.
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Threshold). 8 Peptides matched to identified protein/ fingerprint masses searched. 9 Sequence coverage of the matched peptides. 10 (R)oot (M)ean (S)quare error in ppm of predicted versus apparent peptide mass (m/z). 11 Database. 12 Corresponding accession number of identified proteins. 13 Link to sequences and online resources (Ensembl and TIGR only). Click here for file [ http://www.biomedcentral.
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Rther decreased the yolk and are recommended for 2D gel electrophoresis. The samples were frozen in liquid nitrogen or processed directly for Western blotting or 2D gel electrophoresis.Due to the lack of calcium the cells dissociate during the deyolking. Addition of 2.7 mM CaCl2 to the deyolking buffer preserves bigger cell clusters or cell sheets. Deyolking was only slightly less efficient in the
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Rther decreased the yolk and are recommended for 2D gel electrophoresis. The samples were frozen in liquid nitrogen or processed directly for Western blotting or 2D gel electrophoresis.Due to the lack of calcium the cells dissociate during the deyolking. Addition of 2.7 mM CaCl2 to the deyolking buffer preserves bigger cell clusters or cell sheets. Deyolking was only slightly less efficient in the
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Expression profile of glioblastoma multiforme invasive phenotype points to new therapeutic targets. Neoplasia 2005, 7:7?6. Zagzag D, Salnikow K, Chiriboga L, Yee H, Lan L, Ali MA, Garcia R, Demaria S, Newcomb EW: Downregulation of major histocompatibility complex antigens in invading glioma cells: stealth invasion of the brain. Lab Invest 2005, 85:328?41. Camby I, Belot N, Rorive S, Lefranc F, Mau