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Cs. Disregarded identifications are marked by "(X)". 4 Description of protein function or name of homologous proteins. 5 Basis for the description: TIGR Description as assigned by TIGR zfin Protein/gene name assigned by zfin. Vega Protein/gene name assigned by Vega. NCBI Protein/gene name reported at NCBI REFSEQ Protein/gene name from Refseq. BLAST close homologue based on BLAST-search. Ensembl ch
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Gested with trypsin as described previously [16]: after washing with water, water was removed and gel pieces were shrunk by dehydration with 50 acetonitrile for 15 min. Acetonitrile was removed and the proteins were reduced with 50 10 mM dithiothreitol in 100 mM NH4HCO3 for 30 min at 56 . The solution was removed, gel pieces were dehydrated as before and alkylated with 55 mM iodoacetamide in 1
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E in the treated group compared with control was analyzed by one-way ANOVA followed by Bonferroni's post hoc analysis. *Significantly different compared with PBS-treated control (P
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Nd on the abundance of the target protein. If the yolk is not removed manually, then only 1 or 2 embryos (50?00 ) can be loaded per lane on a gel to avoid overloading effects due to yolk protein. This limits the sensitivity for cellular proteins. The deyolking method enabled us to load significantly more embryos and therefore the signal from specific cellular proteins was increased.Figure 3 demon
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More than 95 of the identifications. We therefore routinely perform searches on MSDB and Ensembl databases. The TIGR database serves as an additional resource for samples which otherwise cannot be identified despite high quality spectra. Both the Ensembl as well as the TIGR project gather a rich pool of information regarding the database entries, such as protein name, homologies, links to gene in
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R 100 mg/kg Triphala 5 times a week. Our results are consistent with previous studies where Triphala was shown to be effective in suppressing the growth ofPage 10 of(page number not for citation purposes)BMC Cancer 2008, 8: http://www.biomedcentral.com/1471-2407/8/. ' .' .' .'0 0.5 1 2 4of cells with DCF fluorescenceS (5. 7KU 7U SS 6HU16 12 8 43 53 FOHDYHGFWLQTPL treatment (hours)1 P0 73/ J PO0.'
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Is, blotting and detection was performed essentially as described in [13]. Briefly, 10 SDS-gels (10 ?10 cm) were run, semi-dry-blotted onto PVDF-membrane as described in the manual (Immobilon P, Millipore), stained for 5 min with Ponceau S, blocked 1 h in 5 nonfat dry milk in PBST (0.5 Tween-20 in PBS), incubated over night at 4 with primary antibody in blocking buffer, washed 30 min with 4 ch
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Olved in an appropriate buffer so that it stays in the supernatant during low speed centrifugation.Figure 5 Comparison of database performance for MS-identification Comparison of database performance for MS-identification. Spots were analysed by MALDI-TOF and subsequently searched against the MSDB, Ensembl and TIGR database. The diagram depicts the number of validated positive identifications by t